rtp [2018-09-07 13:20:18]

replication terminator protein

locus: BSU18490

pI: 9.78

mw: 14.38 kDa

protein length: 122 aa

gene length: 366 bp

function: replication termination

product: replication terminator protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]

Gene

Coordinates: 2,017,886 → 2,018,254

The protein

Catalyzed reaction/ biological activity

• binds to nine termination sites spanning ∼430 kb of the replication terminus region [Pubmed|9642188]

Structure

• [PDB|1F4K] (complex with DNA), [PDB|1BM9]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)
• forms a single focus, clusters of foci, or multiple foci spanning the bilobed nucleoid, consistent with the terminus region [Pubmed|25071173]

Expression and Regulation

Operons

• genes: [gene|4F8E1DEB466D24E13E886BEDB5E3F88226BA9386|rtp]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE18490 (Δ[gene|4F8E1DEB466D24E13E886BEDB5E3F88226BA9386|rtp]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATATGCACTCCTTCTT, downstream forward: _UP4_TAATAGAAACAAACACCCGG
• BKK18490 (Δ[gene|4F8E1DEB466D24E13E886BEDB5E3F88226BA9386|rtp]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATATGCACTCCTTCTT, downstream forward: _UP4_TAATAGAAACAAACACCCGG

References

Reviews

• 19298368

Original publications

• 7746150,10209741,19820088,2498294,2115089,1784827,7934839,9642188,7476199,10064700,8676392,15657033,10931283,25071173,11313334